Extended Data Fig. 3: Paqr4 overexpressing in adipocytes induces insulin resistance upon HFD feeding.
From: PAQR4 regulates adipocyte function and systemic metabolic health by mediating ceramide levels
(a) Immunofluorescence staining of Perilipin (red) and Mac2 (macrophage marker, green) in different fat pads (n = 3). Scale bar 200 µm. (b) Inflammatory gene expressions in BAT at week 3 (WT, n = 12; Paqr4ad, n = 14). (c) Trichrome staining indicated enhanced adipose fibrosis in Paqr4ad mice at week 10 (n = 3). Scale bar 200 µm. (d-e) Dox-dose dependent effects of hyperglycaemia and hyperinsulinaemia in Paqr4ad mice (WT, n = 6; Paqr4ad, n = 7). (f-g) Impaired glucose tolerance and insulin−stimulated glucose disposal in Paqr4ad mice at week 2-3 (WT, n = 14; Paqr4ad, n = 12). (h-i) Dox-dose dependent effects of glucose tolerance in Paqr4ad mice (WT, n = 6; Paqr4ad, n = 7). (j-k) Impaired insulin signalling as accessed by phospho-Akt (Ser473) in various tissues from Paqr4ad mice fed dox-HFD for 6 weeks (WT, n = 3; Paqr4ad, n = 4). Data shown as mean ± SEM and analysed by two-tailed unpaired t-test (b, d-e, i) and two-way ANOVA followed by Holm-Sidak multiple-comparison test (f-h, k).
