Extended Data Fig. 1: PAQR4 is an important player in regulating adipose tissue function.
From: PAQR4 regulates adipocyte function and systemic metabolic health by mediating ceramide levels
Gene expression of Class I Paqr’s in less expanded adipose tissues (AT), including gonadal (gWAT), subcutaneous (sWAT), mesenteric white adipose tissues (mWAT), and brown fat (BAT) from obese mice, compared to massively expanded AT from morbidly obese mice, in males (a) and females (b) (n = 3). -Log2 (FC) and P-values are shown. FC, fold change. (c) Tissue expression of Paqr4 in chow-fed mice (n = 6). (d) HFD feeding increases Paqr4 expression in adipose tissues but not heart (n = 10). (e) Paqr4 is specifically induced in adipose tissues in Paqr4ad mice fed dox chow for 1 week (n = 4). (f-g) Time course of lean mass and percentage of lean mass (WT, n = 8; Paqr4ad, n = 10). (h-i) CT scan of WT and Paqr4ad mice. ROI of gWAT, sWAT and BAT were highlighted, and the fat pad volume was measured (n = 5). (j) Cold-acclimated Paqr4ad mice display hypothermia upon fasting (WT, n = 7; Paqr4ad, n = 6). (k) Overlapping glucose tolerance test prior to dox induction (WT, n = 7; Paqr4ad, n = 6). (l-m) Impaired glucose tolerance and insulin sensitivity in Paqr4ad mice (WT, n = 7; Paqr4ad, n = 6). Data shown as mean ± SEM and analysed by two-tailed unpaired t-test (d-e, i) and two-way ANOVA (f-g, j-m).
