Extended Data Fig. 2: Paqr4 overexpressing in adipocytes reduced weight gain upon HFD feeding.
From: PAQR4 regulates adipocyte function and systemic metabolic health by mediating ceramide levels
Mice were fed dox-HFD (600 mg/kg except in the dox-response assays). (a) Dox-dose dependent of Paqr4 induction in gonadal fat pads in Paqr4ad mice (WT, n = 6; Paqr4ad, n = 7). (b) Paqr4ad mice are smaller. (c-f) Body weight, fat mass, lean mass, percentage of lean mass and tissue mass of WT and Paqr4ad mice in response to different dox doses (WT, n = 6; Paqr4ad, n = 7). (g) Elevated serum ALT levels in Paqr4ad mice at week 10 (n = 6). (h) Respiratory exchange ratio (RER) assessed in mice fed dox-HFD for 2 weeks (n = 6). (i-j) Substrate oxidation calculated from RER and VO2 (n = 6). (k-o) VO2, VCO2, energy expenditure (EE), physical activity and food intake assessed in mice fed dox-HFD for 2 weeks (n = 6). Data shown as mean ± SEM and analysed by one-way ANOVA followed by Holm-Sidak multiple-comparison test (a), two-way ANOVA (c), and two-tailed unpaired t-test (d-g, h-n).
