Extended Data Fig. 4: Glycolytic activity drives oxygen consumption rates following GLS depletion.
From: Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health
a. Oxygen consumption rates (OCR) during mitostress tests in siC or siGLS human adipocytes co-transfected with siC or siUCP1 (left). Data (six replicates per condition, repeated two times) were compared for basal and maximal respiratory capacity. Results were compared using two-way ANOVA and Tukey’s post-hoc test and p values are shown (right). b. OCR during mitostress tests in siC or siGLS human adipocytes incubated with or without glutamine for 48 hours (left). Data were compared for basal and maximal respiratory capacity. Results were compared using two-way ANOVA and Tukey’s post-hoc test and the p value is shown (right) (six replicates per condition, repeated three times). c. OCR during mitostress tests in siC or siGLS human adipocytes (left) incubated with or without glucose. Data were compared for basal and maximal respiratory capacity. Results were compared using two-way ANOVA and Tukey’s post-hoc test and p values are shown (right) (six replicates per condition, repeated two times). d. OCR during mitostress tests in siC or siGLS human adipocytes (left) incubated with or without 2-deoxy-D-glucose (2DG). Data were compared for basal and maximal respiratory capacity. Results were compared using two-way ANOVA and Tukey’s post-hoc test and p values are shown (right) (six replicates per condition, repeated two times). e. OCR during mitostress tests in siC or siGLS human adipocytes (left) incubated with or without UK5099. Data (repeated two times) were compared for basal and maximal respiratory capacity. Results were compared using two-way ANOVA and Tukey’s post-hoc test and p values are shown (right) (six replicates per condition, repeated two times). f. OCR during mitostress tests in siC or siGLS human adipocytes (left) incubated with or without sodium pyruvate. Data (repeated two times) were compared for basal and maximal respiratory capacity. Results were compared using two-way ANOVA and Tukey’s post-hoc test and p values are shown (right) (six replicates per condition, repeated two times). Data in all panels show mean ± S.E.M. Abbreviations: FCCP = carbonylcyanide-p-trifluoromethoxyphenylhydrazone, O = oligomycin, R/A = rotenone/antimycin A.
