Fig. 3: Adipocyte GLS inhibition promotes glucose utilization and oxidative metabolism.
From: Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health
a, OCRs during mitostress tests in siC (black) or siGLS (green) human adipocytes (left). Data (12 replicates per condition, experiment repeated at least three times) were compared for basal respiration and maximal respiratory capacity using Student’s t-test (right). b, Metabolic fuel flex tests in siC or siGLS human adipocytes. Data (12 replicates per condition from more than three experiments) displaying the drop in OCR from baseline after drug injection (UK5099 in left or etomoxir in right) were calculated and compared using Student’s t-test. c, Glucose uptake in human adipocytes transfected with siC or siGLS (four replicates per condition, repeated twice). Data were compared using Student’s t-test. d, Intracellular levels of glucose-6-phosphate (G6P), fructose-6-phosphate (F6P), pyruvate and lactate in human adipocytes transfected with siC or siGLS (six replicates per condition). Data were compared using Student’s t-test. e, ECAR during glycostress tests in siC or siGLS human adipocytes (left). Data (12 replicates per condition, repeated more than three times) were compared for glycolysis and glycolytic (gly.) capacity (right) using Student’s t-test. f–h, Glucose-13C incorporation in pyruvate (f), secreted lactate (g) and TCA intermediates (h) in human adipocytes transfected with siC or siGLS (seven replicates per condition). Data were compared using Student’s t-test. Data in all panels show mean ± s.e.m. Relevant P values are shown. 2DG, 2-deoxy-d-glucose; α-KG, alpha-ketoglutarate; FCCP, carbonyl cyanide-4-(trifluoromethoxy)phenylhydrazone; G, glucose; norm., normalized; O, oligomycin; R/A, rotenone/antimycin A; rel., relative.
