Fig. 5: Pharmacological Gls inhibition in male and female mice reduces fat mass and induces adipocyte Ucp1 expression in inguinal WAT.
From: Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health
a, Body weight changes in male and female mice fed chow (CD) (n = 9 for each gender) or a HFD, treated either with vehicle (n = 12 for each gender) or with 200 mg kg−1 of CB-839 (n = 12 males and n = 10 females) administered daily by gavage for 19 days. Data were analysed by two-way ANOVA and Tukey’s post hoc test. b, The weights of one pad out of the two pads of inguinal (iWAT), one pad out of the two pads of epigonadal (eWAT) and mesenteric (mWAT) WAT as well as BAT were measured in the male and female mice described in a. Data were analysed by one-way ANOVA and Tukey’s post hoc test. c, Ucp1 gene expression profiles were analysed in iWAT samples obtained from the male and female mice described above. Data are presented as min–max (median) and were compared using non-parametric (Kruskal–Wallis) one-way ANOVA. d, The circulating levels of insulin were measured and the Homeostatic Model Assessment-Insulin Resistance (HOMA-IR) index was calculated in male and female mice fed CD (n = 9 males and n = 8 females) or HFD, treated either with vehicle (n = 12 for each gender) or with CB-839 (n = 12 males and n = 10 females) as described above. Data were analysed by one-way ANOVA and Dunnett’s post hoc test. Data in b and d show mean ± s.e.m. The P values are displayed only for the comparison between HFD treated with either vehicle or CB-839.
