Fig. 6: Adipocyte Gls depletion promotes a browning phenotype in inguinal WAT.
From: Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health
a, Gls mRNA expression in adipose depots and different organs from Glsfl/fl (n = 8 for adipose tissues (iWAT, eWAT and BAT) and n = 5 for other organs) and GlsAdipoqCre (n = 5) mice. b, Gls mRNA expression in intact WAT, mature adipocytes (MA) and the stromal vascular fraction (SVF) in the two genotypes (n = 3 per condition). c, Gls protein expression in mature adipocytes isolated from iWAT of Glsfl/fl and GlsAdipoqCre mice (two mice per condition). d, GLS activity in iWAT and eWAT of Glsfl/fl and GlsAdipoqCre mice (n = 3 per condition). e, Data from mice in a displaying the levels of glutamine (gln), glutamate (glu) and the glutamine-to-glutamate ratio in iWAT of Glsfl/fl (n = 8) and GlsAdipoqCre (n = 5) mice. f, Body weight of Glsfl/fl (n = 8) and GlsAdipoqCre (n = 5) mice. g, Fat and lean body mass determined by magnetic resonance imaging (MRI) in Glsfl/fl (n = 8) and GlsAdipoqCre (n = 5) mice. h, Weight of iWAT and eWAT of Glsfl/fl (n = 8) and GlsAdipoqCre (n = 5) mice. i, Fat cell size of iWAT and eWAT (five mice per condition) measured as described in the Methods. Boxplots display the median and the 1.5× IQR method is used to determine the length of the whiskers. j, Representative haematoxylin and eosin staining of iWAT and eWAT with a specified area magnified (to the right of each image). Scale bars represent 100 μm for the images and 20 μm in the magnified areas. k, Representative haematoxylin and eosin staining of BAT with a determined area magnified (to the right side of the images). Scale bars same as in j. a,b and d–h show mean ± s.e.m. Data in a, b and d–i were compared using Student’s t-test. Relevant P values are shown. eWAT, epigonadal WAT; iWAT, inguinal WAT; Rel., relative.
