Fig. 7: Subclustering of mouse adipocytes identifies a distinct beige subpopulation within the iWAT of GlsAdipoqCre mice.
From: Reduced adipocyte glutaminase activity promotes energy expenditure and metabolic health
a, Gls gene expression for each cell population in the single-nucleus RNA sequencing dataset from inguinal WAT (iWAT) of Glsfl/fl and GlsAdipoqCre mice (n = 5 for each genotype). b, UMAP projection of adipocytes in iWAT of Glsfl/fl and GlsAdipoqCre mice. c, Proportion of adipocyte subpopulations in iWAT of Glsfl/fl and GlsAdipoqCre mice. d, Top marker genes across the three identifiable subpopulations in iWAT presented in a dot plot where circle sizes and colours are proportional to the detection rate and expression of the marker genes, respectively. e, Gls gene expression for each cell population in the single-nucleus RNA sequencing dataset from BAT of Glsfl/fl and GlsAdipoqCre mice. f, UMAP projection of adipocytes in BAT. g, Proportion of adipocyte subpopulations in BAT of Glsfl/fl and GlsAdipoqCre mice. h, Ucp1, Slc25a42 and Cfd mRNA expression of brown adipocyte and inguinal white adipocyte of Glsfl/fl and GlsAdipoqCre mice. i, Representative microphotographs of iWAT displaying immunofluorescence signal of TOM20, COX4 and UCP1 protein in Glsfl/fl and GlsAdipoqCre mice. Hoechst was used to stain nuclei. Scale bars, 50 μm. COX4, cytochrome C oxidase subunit; Cfd, complement factor D; Mt, metallothionein 1A; Slc25a42, solute carrier family 25 member 42; TOM20, translocase of outer mitochondrial membrane 20; UMAP, uniform manifold approximation and projection.
