Extended Data Fig. 4: Validation of chemogenetic manipulation. | Nature Metabolism

Extended Data Fig. 4: Validation of chemogenetic manipulation.

From: A subset of dopamine receptor-expressing neurons in the nucleus accumbens controls feeding and energy homeostasis

Extended Data Fig. 4

a, Experimental scheme of chemogenetic manipulation. b, Validation of virus expression in Serpinb2-Cre, Tac2-Cre and Drd1-Cre mice. Scale bar, 500 μm. One technical replicate of three biological replicates. c, cFos induction after intraperitoneal injection of ligand CNO in mCherry-expressing, hM3Dq-mCherry-expressing and hM4Di-mCherry-expressing mice. The ratio of cFos+/mCherry+ cells in all mCherry+ cells was calculated and shown on the right panel. Scale bar, 100 μm. 4 sections from 4 mice per group. One-way ANOVA test, F2,9 = 64.56. Data are represented as mean ± SEM.

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