Extended Data Fig. 5: Serpinb2+ neuronal activity does not affect locomotion, drug seeking, social, anhedonic or anxiety behavior.
a,b, Open field test for the effect of Sepinb2+ neuronal activation (hM3Dq) (a) or inhibition (hM4Di) (b) on the total distance traveled in the 1-hour post-treatment period after chemogenetic manipulation of Sepinb2+ neurons (left) or the distance traveled in 5-min time bin (right) (a, mCherry, 8 mice; hM3Dq, 7 mice; b, 8 mice per group). left, two-tailed, unpaired t-test, t13 = 0.0626 (a), t14 = 0.7754 (b); right, two-way RM ANOVA, F1,13 = 0.0039 (a), F1,14 = 0.6012 (b). c, Left, illustration of the two-chamber CPP paradigm. Right, CPP with chemogenetic activation (hM3Dq) or inhibition (hM4Di) of Sepinb2+ neurons. CPP scores were calculated by subtracting the time spent in the preconditioning phase from the time spent in the postconditioning phase. n = 8 mice per group. Two-tailed, unpaired t-test, t14 = 0.2303 (left), t14 = 0.0863 (right). d, The same as in panel c except conditioned with cocaine. n = 8 mice per group. Two-tailed, unpaired t-test, t14 = 0.8196 (left), t14 = 0.4226 (right). e, Sucrose preference test for the effect of Sepinb2+ neuronal activation (hM3Dq) (left) or inhibition (hM4Di) (right). n = 9 mice per group. Two-tailed, unpaired t-test, t16 = 0.1470 (left), t16 = 0.1821 (right). f,g, 3 chamber social interaction test for the effect of Sepinb2+ neuronal activation (hM3Dq) (top) or inhibition (hM4Di) (bottom). n = 8 mice per group. Two-tailed, unpaired t-test, t14 = 0.0142 (f, left), t14 = 1.200 (f, right), t14 = 0.1427 (g, left), t14 = 0.6685 (g, right), h,i, Elevated plus maze test for the effect of Sepinb2+ neuronal activation (hM3Dq) (h) or inhibition (hM4Di) (i) on the time spent (left) or distance traveled (right) in open arm and closed arm of the 5-min post-treatment period after chemogenetic manipulation of Sepinb2+ neurons. n = 8 mice per group. Two-tailed, unpaired t-test, t16 = 1.010 (h, left, open arm), t16 = 0.0556 (h, left, closed arm), t16 = 0.2663 (h, right, open arm), t16 = 0.3168 (h, right, closed arm). t14 = 0.1410 (i, left, open arm), t14 = 0.1252 (i, left, closed arm), t14 = 0.8526 (i, right, open arm), t14 = 0.0247 (i, right, closed arm). Data are represented as mean ± SEM.
