Fig. 6: Ablation of Serpinb2+ neurons decrease bodyweight gain by reducing food consumption and increasing energy expenditure.
a, FISH and quantification verify Serpinb2+ neuron ablation after AAV-DIO-taCasp3 injection. Scale bar, 100 μm. One technical replicate of three biological replicates. Unpaired t-test, t4 = 12.99. b, Serpinb2+ neuron ablation decreased 3 h total food consumption by mice. n = 8 mice per group. Unpaired t-test, t14 = 5.843. c, Serpinb2+ neuron ablation has a long-term effect on bodyweight (BW) loss. n = 9 mice per group. Two-way ANOVA, Šídák’s test (left), F1,16 = 15.78; unpaired t-test (right), t16 = 5.428. d, Oxygen consumed (VO2) by GFP-expressing control (n = 8) and taCasp3 (n = 8) mice fed with chow. Each symbol represents the mean of eight individual mice (left); data are presented as mean ± s.e.m. (right). Unpaired t-test, t14 = 3.692 (light), t14 = 5.354 (dark). e, Carbon dioxide consumed (VCO2) by GFP-expressing control (n = 8) and taCasp3 (n = 8) mice fed with chow. Each symbol represents the mean of eight individual mice (left); data are presented as mean ± s.e.m. (right). Unpaired t-test, t14 = 2.575 (light), t14 = 3.799 (dark). f, Energy expenditure of mice by GFP-expressing control (n = 8) and taCasp3 (n = 8) mice fed with chow. Each symbol represents the mean of eight individual mice (left); data are presented as mean ± s.e.m. (right). Unpaired t-test, t14 = 3.462 (light), t14 = 5.028 (dark). g, Cumulative food consumption from baseline to day 3. GFP-expressing control (n = 7) and taCasp3 (n = 7) mice fed with chow. Each symbol represents the mean of seven individual mice (left); data are presented as mean ± s.e.m. (middle, right). Two-tailed, unpaired t-test, t12 = 4.969 (middle), t12 = 0.4510 (light), t12 = 3.534 (dark).
