Extended Data Fig. 1: The markers of D1-MSN subtypes in NAcSh do not overlap with Drd2.
a, ISH images showing the spatial distribution of Spon1, Stard5, Tac2 and Serpinb2 markers of certain D1-MSN subtypes, in mouse NAc. Boxed regions in left panels are enlarged and shown in the right panels. The data are from the Allen Mouse Brain Atlas. Scale bars, 500 µm. b, smFISH confirms the expression of D1-MSN subtype-specific markers in the NAcSh do not overlap with Drd2. Boxed regions in left panels are enlarged and shown in the right panels. Three independent experiments were performed with similar results. Scale bar, 50 µm. c, The percentage of NAcSpon1, NAcStard5, NAcTac2 and NAcSerpinb2 cells overlapping with Drd1+ or Drd2+ cells in the NAcSh. t4 = 52.36 (1st), t4 = 35.21 (2nd), t4 = 73.2761 (3rd), t4 = 85.70 (4th). For b,c, every fourth of 16-µm brain sections was counted. n = 3 mice for all groups. All statistical tests are two-tailed unpaired t-tests. Data are mean ± SEM.
