Extended Data Fig. 3: Comparison of thermogenic, lipolytic, insulin signaling, and cellular signaling proteins in aSAT between SED vs EX.

a) Protein abundance of UCP1 and PRDM16. b) Ratio of phosphorylated ATGL to total ATGL and phosphorylated HSL to total HSL. c) Protein abundance of phosphorylated AKT (Thr308 and Ser473), total AKT, and ratio of phosphorylated AKT to total AKT. P = 0.0132 for pAKTT308:AKT. d) Protein abundance of phosphorylated STAT3 (Tyr705), total STAT3, and ratio of phosphorylated STAT3 to total STAT3. e) Representative blot images from SDS-page Western blot analysis (UCP1, PRDM16) and JESS immunoblot analysis (pAKTT308, pAKTS473, AKT, pSTAT3Y705, STAT3) are presented separately. Sample sizes – SED: n = 16 and EX: n = 16. Data is expressed as mean ± SD.