Extended Data Fig. 6: Independent assessment of tumor growth in pancreas and liver in immunocompetent and Nu/J mice.

(a) Weights of the indicated tissues harvested from immunocompetent C57BL/6J mice where primary, or liver metastatic pancreatic cancer cells were implanted into the pancreas, liver, or subcutaneous space on the flank. Weight of age-matched normal pancreatic and liver tissue is also shown. n=5 (pancreas, primary and normal tissues), n=6 (pancreas, liver met); n=4 (liver), n=3 (subcutaneous) male and female mice; Mean+/− stdev; n.s.- not significant. ***p=0.0001. Also shown (right) is the calculated tumor weight (pancreas and liver) and tumor weight (subcutaneous; s.c.) for these data with tumor weight calculated as the difference in tissue weight between the tumor-bearing organ and the normal age-matched tissue from a non-tumor bearing mouse. For both pancreatic tumors and liver tumors, the normal tissue weight of the pancreas or the liver was subtracted to determine the calculated tumor weight, despite the observation that the entire pancreas was transformed with no macroscopic evidence of normal tissue. Subcutaneous (s.c.) tumors represent actual tumor weight. mean+/− stdev; ***p=0.0038. Multiple comparisons were done (right panel) using 2way ANOVA and corrected for multiple comparisons using the Tukey test; cell line effect (p=0.0520), location effect (p=0.0005). (b) Calculated tumor weight represented as the difference in tissue weight between tumor-bearing organ and normal age-matched tissue from non-tumor bearing age-matched immunocompromised nude (NU/J) mice; (n=3 (all samples except normal tissues (n=4)) mean+/− stdev is reported (c) Tissue weight from Fig. 2h, normalized to mouse body weight. (n=3 (all samples except normal tissues (n=4)) mice/group); ***p=0.0006, *p=0.0119, n.s.- not significant; mean+/− stdev (left); calculated tumor weight (as in (a)) normalized to body weight (n=3-4 mice/group); *p<0.05, n.s.- not significant; mean+/− stdev (right). Comparisons between groups were made using a two-tailed Student’s t-test (a-c). (d) Weight of NU/J mice 4 weeks post-implantation of the indicated cancer cells (primary or liver met; tumor bearing mice) or sham injection (non-tumor bearing mice) into the pancreas (left) or liver (right) as indicated; n=4 (non-tumor bearing) or 3 (tumor bearing) mice/group; mean+/− stdev.