Extended Data Fig. 7: Characterization of pancreatic tumor transplants into the pancreas, liver, and lung.

(a) Weights of the indicated tissues harvested from mice where primary pancreatic cancer cells, or liver or lung metastatic pancreatic cancer cells were implanted into the pancreas (pancreatic tumor), liver (liver+tumor), or lung (lung+tumor). Tissue weight +/− stdev. n=3-5 mice. (b) Calculated tumor weight (pancreas, liver, and lung) and tumor weight (subcutaneous) corresponding to data shown in Fig. 2k; tumor weight is calculated as the difference in tissue weight between the tumor-bearing organ and normal age-matched tissue from a non-tumor bearing mouse. For pancreatic tumors, liver tumors, and lung tumors the normal tissue weight of the pancreas, the liver, or the lung was subtracted to determine the calculated tumor weight, despite the observation that the entire pancreas was transformed with no macroscopic evidence of normal tissue. Subcutaneous tumors (s.c.) represent actual tumor weight. mean+/− stdev; *p<0.05, *p<0.01, ***p< 0.001. n=3-5 mice. Comparisons between individual groups were made using a two-tailed Student’s t-test. Multiple comparisons were done using 2way ANOVA and corrected for multiple comparisons using the Tukey test; cell line effect (p=0.0256), location effect (p < 0.0001). (c) Macroscopic images of liver (left) or lung (right) four weeks post injection of cancer cells derived from a primary pancreatic tumor (primary >) or from a liver or lung metastasis (liver met > or lung met >, respectively) into the indicated organ. N- normal tissue; T- tumor; arrows indicate tumor nodules in the lung. Each macroscopic image is from one mouse. (d-f) Whole mount H&E-stained liver or lung tissue from mice where primary pancreatic cancer cells (d), liver (e), or lung (f) metastatic cancer cells were implanted into the indicated site. Scale bars are indicated in the figure panels; higher magnification images are shown for smaller tumors; tumors in the liver are indicated. Histological images are shown for each individual mouse (g) Macroscopic images of lung four weeks post tail vein injection of pancreatic liver metastasis-derived cells. (h) Weights of age-matched normal lung (n=2 mice) or weights of lungs with tumors (n=3 mice) from female mice described in (g). Mean +/− stdev (left); Tumor weight is calculated as the difference in tissue weight between the tumor-bearing lung and normal age-matched lung from a non-tumor bearing mouse. Mean +/− stdev (right). (i) Whole mount H&E-stained lung tissue from mice injected with pancreatic liver metastases via tail vein. Scale bar- 2 mm. Higher magnification is shown on the bottom; scale bar- 100 μM. Histological image is from each mouse and is an independently repeated experiment from an independently derived cell line to accompany data shown in Fig. 2k and Extended Data Fig. 7b.