Extended Data Fig. 5: Assessment of proliferation and cell death in tumors.

(a-c) Ki67 staining of tumor tissue derived from primary or liver metastatic (Liver Met) pancreatic cancer cells that were implanted into the pancreas (left) or the liver (right) (a) or staining of tumor tissue from primary or lung metastatic (Lung Met) pancreatic cancer cells implanted into the pancreas (b), or lung (c) as indicated. scale bar, 50 μm. (d) Immunohistochemistry stain for BrdU in a representative tumor obtained from primary or liver metastatic pancreatic cancer cells injected in either the pancreas (left) or liver (right). scale bar, 20 μm. (e-g) Immunohistochemistry staining for cleaved caspase 3 in tumor sections derived from primary, liver, or lung metastatic pancreatic cancer cells injected in either the pancreas (e), liver (f) or lung (g). Representative region per section is shown with higher magnification image shown in the bottom right inset; scale bar for panels e-g: lower magnification- 200 μm, inset- 20 μm. For panels a-f, each image is a representative region from full scan of the entire section from 1 mouse.