Extended Data Fig. 5: The neutrophils from Lifrfl/fl;Alb-Cre mice have a lower ability to promote hepatocyte proliferation.
a. Immunofluorescence staining of neutrophil elastase (NE, green) and Hgf (red) on liver sections from Lifrfl/fl and Lifrfl/fl;Alb-Cre mice at 72 hours after PHx. Scale bars, 100 μm. Representative results from one of three independent experiments are shown. b. Immunofluorescence staining of Ki67 (overlay with DAPI staining) and percentage of Ki67-positive cells in primary mouse hepatocytes cultured for 48 hours with the conditioned medium of liver-infiltrating neutrophils purified from Lifrfl/fl and Lifrfl/fl;Alb-Cre mice at 72 hours after PHx. Scale bars, 100 μm. n = 5 biological replicates. c. Immunofluorescence staining of Ki67 (overlay with DAPI staining) and percentage of Ki67-positive cells in primary mouse hepatocytes cultured for 48 hours with the conditioned medium of blood neutrophils purified from Lifrfl/fl and Lifrfl/fl;Alb-Cre mice at 72 hours after PHx. Scale bars, 100 μm. n = 5 biological replicates. d. Immunoblotting of p-Met, Met, p-Erk, Erk, and Gapdh in the livers of Lifrfl/fl and Lifrfl/fl;Alb-Cre mice at 72 hours after PHx. Representative results from one of three independent experiments are shown. Statistical significance in b and c was determined by a two-tailed unpaired t-test. Error bars are s.e.m.
