Extended Data Fig. 6: Knockdown of HGF in AdHL-60 cells.
a. Giemsa staining of HL-60 and all-trans retinoic acid (ATRA)-differentiated HL-60 (AdHL-60) cells. Scale bars, 50 μm. b. Flow cytometry plots of CD11b in HL-60 and AdHL-60 cells. c. Giemsa staining of control and HGF-knockdown HL-60 cells with or without ATRA-induced differentiation. Scale bars, 50 μm. d. Flow cytometry plots of CD11b in control and HGF-knockdown HL-60 cells with or without ATRA-induced differentiation. e. qPCR of HGF in control and HGF-knockdown AdHL-60 cells. n = 3 biological replicates. f. Flow cytometry plots and quantification of HGF in control and HGF-knockdown AdHL-60 cells. n = 3 biological replicates. Statistical significance in e and f was determined by a two-tailed unpaired t-test. Error bars are s.e.m.
