Extended Data Fig. 9: Cxcl1 facilitates the recruitment of neutrophils to the liver after hepatectomy.
a. Immunofluorescence staining of neutrophil elastase (NE, red), Cxcr2 (green), and Hgf (cyan) in blood neutrophils from wild-type mice at 72 hours after PHx. Scale bars, 5 μm. Representative results from one of three independent experiments are shown. b, c. Schematic of the experimental design (b): at 40 hours after PHx, CD45.2 mice were treated with isotype IgG or anti-Cxcl1. At 62 hours after PHx, CD45.1 neutrophils were adoptively transferred to the antibody-treated mice. The liver infiltration of CD45.1 neutrophils was analyzed at 4 hours after adoptive transfer (c). n = 6 mice. Statistical significance in c was determined by a two-tailed unpaired t-test. Error bars are s.e.m.
