Extended Data Fig. 1: Long-term characterization of aged mice 40-weeks after non-β-cell ablation.
From: Regulated and adaptive in vivo insulin secretion from islets only containing β-cells
a. Immunofluorescence on pancreas tissue sections from control (Ctrl) or DT-treated mice analyzed 40 weeks post-ablation (wpa). INS: red; GCG: green (left); INS: red; SST: green (middle); INS: red; PPY: green (right). Scale bar: 20 µm. b. Quantification of GCG+, SST+, and PPY+ cells in Ctrl (black) or DT (red) mice 40wpa. GCG+ and SST+ (Ctrl n = 7, DT n = 5) cells were scored in the dorsal region of the pancreas; PPY+ (Ctrl n = 3, DT n = 5) cells were scored in the ventral region of the pancreas. P-values Ctrl vs DT: GCG + P = 0.0025, SST + P = 0.0025, PPY + P = 0.036. c. Pancreatic insulin content (ng/mg) of Ctrl (black, n = 6) and DT (red, n = 6) mice 40wpa. P-value Ctrl vs DT = 0.937. Only male mice were used. All data are shown as mean ± sem. All statistical tests are two-tailed Mann-Whitney test. Source data is available.
