Extended Data Fig. 2: Related to Fig. 1: Examples of extracted ion chromatograms.
From: Efferocytosis drives a tryptophan metabolism pathway in macrophages to promote tissue resolution
a, Reversed phase separation and mass spectrometry detection of 12 neat tryptophan metabolite standards (10 ng) individually analyzed. Each trace shows ion signal at a given mass-to-charge ratio (m/z) as the compounds elute from the Atlantis T3 reversed phase column. b, Reversed phase separation on an Atlantis T3 reversed phase column and mass spectrometry detection of tryptophan metabolites from an AC- wild type sample. Each trace shows ion signal at a given mass-to-charge ratio (m/z) with a mass tolerance of 5 ppm. For both sets of chromatograms, data are shown for: (i) 2−picolinic acid), (ii) nicotinic acid, (iii) nicotinamide, (iv) quinolinic acid, (v) nicotinamide adenine dinucleotide (NAD)+, (vi) serotonin, (vii) DL-kynurenine, (viii) N-formylkynurenine, (ix) L-tryptophan, (x) tryptamine, (xi) kynurenic acid, and (xii) anthranilic acid. The normalization level (NL) indicates the intensity of the base peak for each spectrum. In b, although more background was observed in the extracted ion chromatogram for anthranilic acid, the anthranilic acid signal is easily differentiated from the background.
