Extended Data Fig. 8: Related to Fig. 8: Systemic and lesional parameters in control and Mϕ-IDO1-iKO BMT Ldlr^−/− mice.

Ldlr^−/− mice were transplanted with BM from Ido1^fl/fl (Control) or Ido1^fl/flCx3cr1cre^ERT2+/− (Mϕ-IDO1-iKO) mice and then fed the Western diet for 16 weeks. One cohort from each group was harvested (Baseline), and the rest of the mice were switched to chow diet, injected with HDAd-LDLR virus, and given tamoxifen. After 7 weeks, the mice were harvested (Regression). a-k, Body weight, total plasma cholesterol, fasting blood glucose, complete blood count (n = 9–10 mice/group). WBC, white blood cell; NE, neutrophils; LY, lymphocytes; MO, monocytes; EO, eosinophils; BA, basophils, RBC, red blood cells; PLT, platelets. l, Immunostaining of IDO1 (red) and Mac2 (green) in regressing aortic root lesions, with quantification of IDO1 MFI in Mac2⁺ and Mac2⁻ areas. Arrows, examples of IDO1-Mac2 co-localization. DAPI was used for nuclear staining. Scale bar, 25 μm (n = 10 mice/group). m, Quantification of lesion area, based on H&E staining of the aortic root lesions (n = 10 mice/group). n, The regressing aortic root lesions of Control and Mϕ-IDO1-iKO groups were immunostained for Mac2 (macrophages; green) and TGF-β1 or IL-10. (red) Arrows, examples of colocalization of Mac2 and TGF-β1 (top) and Mac2 and IL-10 (bottom). DAPI (blue) was used for nuclear staining. Scale bar, 50 μm. o, The total number of Mac2⁺ cells per lesion section was quantified in regressing aortic root lesions (n = 10 mice/group). The data are expressed as mean ± SEM, and significance was determined by one-way ANOVA with Fisher’s LSD post-hoc analysis for panels a-k and m, and by Student’s t-test for panels l and o.