Extended Data Fig. 1
From: Spatial single-cell isotope tracing reveals heterogeneity of de novo fatty acid synthesis in cancer
a) Box plots displaying mean and distribution of normalized intensities of 11 different fatty acids detected by 13C-SpaceM in normoxic and hypoxic cells. The box shows the quartiles of the dataset. Whiskers extend to show the range from Q1 – 1.5 IQR to Q3 + 1.5 IQR, where IQR is the inter quartile distance. The points that do not fall inside this range are considered to be outliers and are shown explicitly. b) Normalized intensities of 12 fatty acids released from total lipids isolated by saponification from normoxic cells and quantified using bulk MS with direct infusion. Error bars reflect standard deviation of the mean calculated from three biological replicates. c) Histogram of the distribution of cell area as determined by imaging for cells cultured in normoxia and hypoxia.
