Extended Data Fig. 6: Hepatotoxicity in LXRα knockout mice fed a western diet.
8-week-old wild-type LXRα+/+ (Wt, N = 8), heterozygous LXRα+/- (Het, N = 19) and homozygous LXRα-/- (Hom, N = 8) mice were placed on a high-fat (42.7% by Kcal), high-sucrose (34.1% by weight), high cholesterol (0.2%) diet (western diet) for 8 weeks then euthanised and blood and tissues were collected for downstream analyses. Unless specified, all mice were assessed for: A: Endpoint weight measurements B-C: Fat and lean mass assessed by Echo MRI at 16 weeks of age. D-G: Serum biochemistry from terminal bleeds. H: Selected organ weights. I-J: Free cholesterol (CHL) and CHL ester measurements in liver lysate normalized to organ weight are shown for N = 8 mice of each genotype. K: Triglyceride (TG) measurements in liver lysate normalized to organ weight are shown for all mice. Representative images of H&E staining are shown in (L). Livers from wild-type (N = 8), heterozygous (N = 12) and homozygous (N = 8) mice were stained for collagen using picrosirius red (PSR) stain and quantified by HALO (M). Representative images of PSR staining are shown in (N). In (A-K, M), analysis was performed using Kruskal–Wallis test with Dunn’s multiple comparison test or ordinary one-way ANOVA with Holm-Šídak multiple comparison, based on the distribution of the data. All data are presented as mean ± SD, except G where mean ± SEM is represented.
