Extended Data Fig. 4: Knockdown of nucleic acid sensors in BMDMs. | Nature Metabolism

Extended Data Fig. 4: Knockdown of nucleic acid sensors in BMDMs.

From: Itaconate drives mtRNA-mediated type I interferon production through inhibition of succinate dehydrogenase

Extended Data Fig. 4

a-d, Expression of a, Ddx58, b, Ifih1, c, Mb21d1, and d, Tlr9 in BMDMs in the presence of a,c, Mb21d1, Ddx58, b,d, Tlr9 and Ifih1 siRNA pretreated with DMSO or TTFA (n=3 mice; LPS 4 h) from 3 independent experiments. e-g, Expression of e, Mb21d1 f, Ifih1, and g, Ddx58 in the presence of Mb21d1, Ifih1, and Ddx58 siRNA in BMDMs pretreated with PBS or ITA (n=2-3 mice; LPS 4 h) from 2 or 3 independent experiments. h-j IFNβ release in BMDMs transfected with h, G3-YSD, i, Poly(I:C), or j, 5′PPP-dsRNA in the presence of h, Mb21d1, i, Ifih1, or j, Ddx58 siRNA (n=3 mice; 4 h) from 3 independent experiments. k, Ifnb1 and l, Tmem173 expression in BMDMs pretreated with DMSO or TTFA in the presence of Tmem173 siRNA (n=3 mice; LPS 4 h). Data are presented as mean ± SEM. P values were calculated using two-way ANOVA for multiple comparisons.

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