Extended Data Fig. 4: Loss of NAMPT does not compromise innate immune response against HSV-1.
From: Cryptic phosphoribosylase activity of NAMPT restricts the virion incorporation of viral proteins
a, NAMPT-KO in HepG2 cells was validated by immunoblotting with indicated antibodies using whole cell lysates. b-c, Heatmap of cytokine gene expression in sgCTL and sgNAMPT HepG2, with Sendai virus (SeV, 30 HA unit/ml) (b) and HeLa cells infected with HSV-1 (MOI = 2) (c). n = 3 for sgCTL HepG2 infected with Sendai virus for ISG15 and IRF7 analysis, n = 3 for sgCTL HepG2 mock group for IRF7 analysis, n = 4 for all the rest analyses. d, The mRNA of indicated inflammatory genes was determined by reverse transcription and real-time PCR using the liver of wildtype (n = 4 for mock, n = 6 for HSV-1 infection), NAMPT-KO (n = 4 for mock, n = 5 for HSV-1 infection) and NAMPT-KO mice with nicotinamide riboside (dose: 400 mg/kg/day, n = 4 for mock, n = 5 for HSV-1 infection) at 3 days post-infection of HSV-1 (2 × 107 PFU, intravenous). ND, not detected. Data in c and d are presented as mean values\(\pm\)SD.
