Extended Data Fig. 4: Characterization of Jurkat-RPS3-Keima and Jurkat-UCK tKO cell lines. | Nature Metabolism

Extended Data Fig. 4: Characterization of Jurkat-RPS3-Keima and Jurkat-UCK tKO cell lines.

From: Glucose limitation protects cancer cells from apoptosis induced by pyrimidine restriction and replication inhibition

Extended Data Fig. 4

a. PCR confirmation of RPS3-Keima allele (left) and immunoblot of FLAG-tagged RPS3-Keima protein (right). b, Immunoblot analysis of Keima processing in Jurkat-RPS3-Keima cells treated with brequinar (300 nM), RTX (10 nM) or Torin1 (150 nM). *RPS3-Keima, **processed Keima. c, Proliferation of UCK2 knockout or UCK1/2 double knockout Jurkat cells treated with brequinar ± uridine (U, 50 μg/ml), n = 3 biological replicates. d, Proliferation of Jurkat-UCK tKO clones treated with PALA (150 μM) ± uridine (50 μg/ml), n = 3 biological replicates. Data are mean ± SD.

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