Extended Data Fig. 1: The effects of pyrimidine/purine synthesis inhibitors and aminopterin on proliferation of cancer cells.
a,b, Dose-response curves for proliferation of Jurkat (a) and Raji (b) cells treated with brequinar or PALA in high and low glucose. c, Uridine-mediated rescue of proliferative defects of Jurkat cells treated with brequinar or PALA. n = 3 biological replicates. d,e, Determination of the IC50 of mizoribine (n = 4 independent experiments in d, n = 3 in e), MPA (n = 5 in d, n = 3 in e) and aminopterin (n = 3) in Jurkat (d) and Raji (e) cells in high and low glucose. f,g, Dose-response curves for proliferation of Jurkat (f) and Raji (g) cells treated with mizoribine, MPA or aminopterin. Data are mean ± SEM except (c; mean ± SD). Statistical significance is determined by two-sided Student’s t-test (d-e), ns: not significant.
