Extended Data Fig. 6: Liver RNA sequencing analysis of anti-ACBP/DBI mAb model.
From: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing’s syndrome
(a) At the end of the corticosterone (CORT) and anti-ACBP/DBI (αACBP) mAb experiment, liver tissue was collected for RNAseq analysis (n=5/group). Genes with a differential expression P-value ≤ 0.05 (Wald test) and abs (fold change) ≥ 2 were selected in (a). (b) Volcano plot of differential genes between isotype + CORT and isotype + Ctrl groups. (c) Volcano plot of differential genes between αACBP + CORT and isotype + CORT groups. Log2 (fold change)| ≥ 1, P-value < 0.05. (d,e) Venn diagrams illustrate overlaps of the transcriptomic CORT effects on isotype and αACBP mAb neutralization. (f) GO enrichment analysis of the genes obtained from the overlap in (d) (58 genes). (g) GO enrichment analysis of the genes obtained from the overlap in (e) (442 genes). Data were analyzed using R software with the DESeq2 package.
