Fig. 5: Passive immunization of mice by neutralizing monoclonal anti-ACBP/DBI mAb prevents the manifestation of Cushing’s syndrome.
From: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing’s syndrome
a, The experimental schedule for passive immunization. Female C57BL/6J mice were treated with CORT (100 μg ml−1 or vehicle control (control) in drinking water, p.o.) for 5 weeks together with ACBP/DBI mAb (αACBP, 5 mg kg−1 body weight, i.p., semiweekly). Isotype was used as the control. Animals were subjected to FST in the fifth week. b, Plasma ACBP was measured by ELISA in the indicated treatment groups (n = 10 per group). c,d, The average food intake (n = 4 cages per group) (c) and body weight (n = 10 per group) (d) was monitored in the indicated groups. e, Facial angles of mice from the indicated groups were measured (n = 3 mice per group). f, Immobility time assessed by FST (n = 10 mice per group). g, The heatmap shows the standardized deviations (z scores) of tissue weight relative to body weight and the quantification of various biochemical parameters across the treatment groups (n = 10, 9, 10 and 10 mice per group). Statistical comparisons were performed by pairwise (two-tailed) Wilcoxon test with false discovery rate (FDR) correction for multiple comparisons (P values are indicated). h–k, GTT (n = 10 mice per group) (h) and ITT (n = 10 mice per group) (j) were monitored in the indicated groups. The P value represents the comparison of areas under the curve (GTT (i) and ITT(k)). The P value represents the comparison of areas under the curve. Statistical comparisons were performed by pairwise Wilcoxon tests with FDR correction for multiple comparisons in the heatmaps (P values are indicated). All dot plots represent mean ± s.e.m. The curves in c and d were longitudinally analysed with type II ANOVA and pairwise comparisons. The data in b, e, f, i and k were analysed using one-way ANOVA with Tukey correction. vWAT, visceral white adipose tissue; iWAT, inguinal white adipose tissue; pWAT, perigonadal white adipose tissue; iBAT, interscapular brown adipose tissue. Created with BioRender.com.
