Fig. 7: Mitigation of CORT-induced metabolic dysregulation in mice under pair feeding by ACBP/DBI neutralization.
From: Pathogenic role of acyl coenzyme A binding protein (ACBP) in Cushing’s syndrome
a, The experimental schedule for the pair-feeding experiment in C57BL/6J female mice receiving anti-ACBP/DBI antibody, isotype control antibody and/or CORT. A pair-feeding protocol was implemented where the average daily food intake of vehicle groups was measured daily to limit the amount of food provided to the other groups. b, Plasma ACBP was measured by ELISA (n = 10 per group). c, Body weight modifications (n = 10 per group) were monitored. d, The heatmap shows the standardized deviations (z scores) of tissue weights relative to body weight and the quantification of various biochemical parameters across the treatment groups (n = 10 mice per group). Statistical comparisons were performed by pairwise (two-tailed) Wilcoxon test with false discovery rate (FDR) correction for multiple comparisons (P values are indicated). e–h, GTT (n = 10 per group) (e and f) and ITT (n = 10 per group) (g and h) are shown (e and g) with the comparison of the areas under the curve (f and h). Statistical comparisons were performed by pairwise Wilcoxon tests with FDR correction for multiple comparison in the heatmaps. All dot plots depict mean ± s.e.m. The weight curves were longitudinally analysed with type II ANOVA and pairwise comparisons. The data in b, f and h were analysed using one-way ANOVA with Tukey correction. vWAT, visceral white adipose tissue; iWAT, inguinal white adipose tissue; pWAT, perigonadal white adipose tissue; iBAT, interscapular brown adipose tissue. Created with BioRender.com.
