Extended Data Fig. 8: Effect of Mertk knockout on metabolism.
From: A feeding-induced myokine modulates glucose homeostasis
a-m, Effect of Mertk knockout on body weight (a), food intake (b), water intake (c), fat mass ratio (d), movement (e), energy expenditure (f), respiratory exchange ratio (RER, g), hepatic glycogen (h), muscle glycogen (i), plasma total cholesterol (j), hepatic cholesterol (k), blood glucose (l), and glucose uptake measured by luciferase activity (m) in mice. The relative area under the curve (AUC) is shown in the inset (l). n = 7 male mice (a-g, j-k), n = 8 male mice (h-i), n = 10 female mice (l). n, Effect of Mertk knockout on translocation of GLUT4 to the plasma membrane in Gastro and eWAT. Left panel shows representative immunostaining results; center and right panels show immunoblots and the corresponding quantification. In these assays, mice were refed for 1 hr after overnight fasting. ATP1A1, a plasma membrane protein, is used as a marker. TCL, total cell lysate; PM, plasma membrane fraction; LDM, low-density membrane fraction. Scale bars, 50 μm. n = 4 biological replicates. o-r, Effect of Mertk knockout on plasma triglycerides (TGs, o), hepatic TGs (p), tissue morphology evaluated by hematoxylin and eosin staining (q), and plasma insulin (r) in mice. cFeimin, cellular feimin. Scale bars, 50 μm. n = 8 male mice. s, Quantification of immunoblots (Fig. 3k) showing AKT activation in liver, Gastro and eWAT extracts from Mertk+/+ and Mertk−/− mice. Mice were refed for 1 hr after overnight fasting. n = 6 biological replicates. t-u, Effect of high-fat diet (HFD) on body weight (t), glucose tolerance test (GTT) and insulin tolerance test (ITT) (u). The relative area under the curve (AUC) is shown in the inset. n = male 8 mice. v, Immunoblots and quantification showing AKT activation in extracts of liver, Gastro and eWAT from male Mertk+/+ and Mertk−/− mice fed with a HFD. Mice were refed for 1 hr after overnight fasting. Quantification of immunoblots is shown on the right. n = 6 biological replicates. w, Plasma Gas6 and Pros1 levels from male Mertk+/+ and Mertk−/− mice, or feimin WT and MKO mice. Mice were refed for 1 hr after overnight fasting. n = 6 mice. Data are shown as mean ± s.e.m. Statistical comparisons were performed using an unpaired two-tailed Student’s t-test (a-d, h-l, n-p and r-w) or two-way ANOVA followed by Tukey’s test (e-g). All individual points and P values are shown.
