Extended Data Fig. 2: Feimin is a feeding-induced myokine.
From: A feeding-induced myokine modulates glucose homeostasis
a, qPCR results showing relative mRNA levels of feimin in different mouse tissues. Gastro, gastrocnemius muscle; eWAT, epidydimal white adipose tissue; BAT, brown adipose tissue. n = 5 mice. b, Immunoblots showing the levels of feimin protein in different tissues from 8-10-week-old male mice. cFeimin, cellular feimin. c, qPCR results showing relative mRNA levels of feimin in different human tissues. n = 3 humans. d, Top panel, generation of feimin−/− mice by CRISPR-Cas9. A deletion of 97 bp was introduced into exon 2 of the feimin gene. Bottom panel, the partial N-terminal sequence of WT feimin and the full sequence of the truncated feimin protein product in feimin−/− mice are shown. The frame-shifted amino acids of feimin generated by the deletion in feimin−/− mice are shown in red. e, Immunoblots showing global knockout of feimin. cFeimin, cellular feimin. f, Generation of feimin intestine-specific knockout (IKO), muscle-specific knockout (MKO), liver-specific knockout (LKO) and adipose tissue knockout (FKO) mice (left panel) and immunoblots showing tissue-specific knockout of feimin (right panel). cFeimin, cellular feimin; Intes, intestine. g, Plasma feimin levels from WT (fl/fl) and muscle-specific feimin knockout (MKO) mice. Samples were collected after overnight fasting (Fasted) and then 30 min after glucose injection (1 g kg−1, Fasted/Glc). 8-10-week-old male mice were used in this assay. n = 8 mice. h, Plasma feimin levels from WT (fl/fl) and muscle-specific feimin knockout (MKO) mice. Samples were collected after 6 hr fasting and then 30 min after insulin injection (1 U kg−1). 8-10-week-old male mice were used in this assay. n = 8 mice. Data are shown as mean ± s.e.m. Statistical comparisons were performed using two-way ANOVA followed by Tukey’s test (g, h). All individual points and P values are shown.
