Extended Data Fig. 10: Kinematic analysis of motor coordination in 3-month-old WT, ATG5 cKO and ATG5:GLUT2 cKO mice.
From: Autophagy regulator ATG5 preserves cerebellar function by safeguarding its glycolytic activity
a,d,g, Y-coordinates of WT (N = 8) (a) ATG5 cKO (N = 11) (d) and ATG5:GLUT2 cKO (N = 6)(g) animals walking on a 12mm-wide beam. b,e,h, Hip, knee and ankle angles of WT (N = 8) (b), ATG5 cKO (N = 11) (e) and ATG5:GLUT2 cKO (N = 6) (h) animals walking on a 12mm-wide beam. c,f,i, Hip, knee, ankle and hindpaw velocities of WT (N = 8) (c), ATG5 cKO (N = 11) (f) and ATG5:GLUT2 cKO (N = 6) (i) animals walking on a 12mm-wide beam. j, PCA analysis based on the y-coordinates, angles, and velocities for 12mm-wide beam. Individual mice are represented as colored circles and dashed lines group mice of similar genotype. k,n,q, Y-coordinates of WT (N = 10) (k), ATG5 cKO (N = 3) (n) and ATG5:GLUT2 cKO (N = 6) (q) animals walking on a 5mm-wide beam. l,o,r, Hip, knee and ankle angles of WT (N = 10) (l), ATG5 cKO (N = 3) (o) and ATG5:GLUT2 cKO (N = 6) (r) animals walking on a 5mm-wide beam. m,p,s, Hip, knee, ankle and hindpaw velocities of WT (N = 10) (m), ATG5 cKO (N = 3) (p) and ATG5:GLUT2 cKO (N = 6) (s) animals walking on a 5mm-wide beam. t, PCA analysis based on the y-coordinates, angles, and velocities for 5mm-wide beam. Individual mice are represented as colored circles and dashed lines group mice of similar genotype. u,v, Variation in knee (u) and hip (v) angles of WT (N = 9), ATG5 cKO (N = 9) and ATG5:GLUT2 cKO (N = 6) animals walking on a 25mm-wide beam. Statistical significance was calculated with two-way ANOVA followed by Bonferroni’s post-hoc test. w,x, Bar graphs showing the duration of stance (w) and swing (x) of WT (N = 9), ATG5 cKO (N = 9) and ATG5:GLUT2 cKO (N = 6) crossing beams of different widths. Statistical significance was calculated with one-way ANOVA followed by Tukey’s post-hoc test. Red arrowheads in (d,e,f,o,p) indicate the points in the step cycle at which the relative positions of the join differ in ATG5 cKOs compared to controls and ATG5:GLUT2 cKOs. In j,k Individual mice are represented as colored circles and dashed lines group mice of similar genotype. All graphs show mean ± SEM. Data presented in Fig. S10b-x show mean as a filled dark line, and SEM as the shaded area around it. n.s.—non-significant; * indicates P ≤ 0.05; ** indicates P ≤ 0.01; *** indicates P ≤ 0.001; **** indicates P ≤ 0.0001.
