Extended Data Fig. 1: Impaired autophagy in ATG5 cKO cerebellum without DCN or interneuron loss.
From: Autophagy regulator ATG5 preserves cerebellar function by safeguarding its glycolytic activity
a, ATG5 ~ ATG12 protein levels in WT and ATG5 cKO cortical lysates. N = 4 mice for each genotype and condition. 1 M: p = 0.0006, 3 M: p = 0.0005. b, LC3 II/LC3 I ratio in WT and ATG5 cKO cortical lysates. N = 3 mice for each genotype for 1 M and N = 4 for 3 M. 1 M: p = 0.013, 3 M: p = 0.004. c, p62 protein levels in WT and ATG5 cKO cortical lysates. N = 3 mice for each genotype for 1 M and N = 4 for each genotype for 3 M. 1 M: p = 0.008, 3 M: p = 0.0002. d, ATG5 ~ ATG12 protein levels in WT and ATG5 cKO cerebellar lysates. N = 4 for each genotype and condition. 1 M: p = 0.0002, 3 M: p = 0.0004. e, LC3 II/LC3 I ratio in WT and ATG5 cKO cerebellar lysates. N = 4 mice for each genotype and condition. 1 M: p = 0.033, 3 M: p = 0.008. f, p62 protein levels in WT and ATG5 cKO cerebellar lysates. N = 4 mice for each genotype and condition. 1 M: p = 0.019, 3 M: p = 0.011. g, GFAP levels in WT and ATG5 cKO cortical lysates. N = 4 mice for each genotype and condition. h, GFAP levels in WT and ATG5 cKO cerebellar lysates. N = 4 mice for each genotype and condition. 3 M: p = 0.008. i,j, Calbindin protein levels in WT and ATG5 cKO cerebellar lysates. N = 4 mice for each genotype and condition. 3 M: p = 0.007. k, WT and ATG5 cKO cerebellum, immunostained for parvalbumin. Scale bar, 200 µm. l, Average volume analysis of WT and ATG5 cKO cerebellum at 3 M based on Nissl staining. N = 5 for WT and N = 4 for ATG5 cKO mice. Two-tailed unpaired t-Test: p = 0.002. m,n, Cell density in the deep cerebellar nuclei (DCN) in WT and ATG5 cKO cerebellum. N = 5 for WT and N = 4 for ATG5 cKO mice. Two-tailed unpaired t-Test. o,p NeuN levels in WT and ATG5 cKO cerebellar lysates. N = 4 mice per genotype. Two-tailed unpaired t-Test. 1 M and 3 M indicate 1 and 3 months, respectively. All graphs show mean ± SEM. Statistical significance in a-j was determined by Two-way ANOVA followed by Holm-Šidák multiple comparisons. n.s.—non-significant; * indicates P ≤ 0.05; ** indicates P ≤ 0.01; *** indicates P ≤ 0.001; **** indicates P ≤ 0.0001.
