Extended Data Fig. 5: The effects of ALDOA depletion are partially ameliorated by culture in low glucose. | Nature Metabolism

Extended Data Fig. 5: The effects of ALDOA depletion are partially ameliorated by culture in low glucose.

From: Targeting aldolase A in hepatocellular carcinoma leads to imbalanced glycolysis and energy stress due to uncontrolled FBP accumulation

Extended Data Fig. 5

a) Intracellular levels of inorganic phosphate (Pi) in shRen, shGPI.213, shGPI.1604 and shGPI.1544 expressing Akt1myr;MycOE;Trp53–/– cells treated with doxycycline (1 µg/mL) for 48hrs. Data are presented as mean values ± SD. Significance was calculated using one-way ANOVA with Dunnett's post-hoc test (n = 4 biologically independent replicates). b) Glycolytic activity of control (shRen) and AldoA knockdown cells (shAldoA) under the indicated glucose concentrations. Cells were analysis using the glycolytic stress test (Seahorse). Data are presented as mean values ± SD (n = 6 parallel wells). c) Proliferation of control (shRen) or AldoA (shAldoA.1280) cells in medium containing ethanol (solvent) and indicated glucose concentrations. Data are presented as mean values ± SD (n = 3 biologically independent replicates).

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