Extended Data Fig. 6: Deletion of GPI rescues the impact of ALDOA deletion in human HCC cells. | Nature Metabolism

Extended Data Fig. 6: Deletion of GPI rescues the impact of ALDOA deletion in human HCC cells.

From: Targeting aldolase A in hepatocellular carcinoma leads to imbalanced glycolysis and energy stress due to uncontrolled FBP accumulation

Extended Data Fig. 6

a) ATP and ADP levels in Akt1myr;MycOE;Trp53–/– cells after deletion of AldoA or after combined deletion of Aldoa and Gpi. Data are presented as mean values ± SD. Significance was calculated using one-way ANOVA with Dunnett's post-hoc test (n = 3 biologically independent replicates). b) Cell viability of human liver cancer cells (HLE, HLF and SNU-387) after deletion of GPI (sgGPI #1/#3) either alone or on combination with ALDOA- (sgALDOA #1, sgALDOA #2). Data are presented as mean values ± SD. Significance was calculated using a two-tailed student’s t-test with FDR correction (n = 3 biologically independent replicates). c) Immunoblot confirming the deletion of GPI (sgGPI #1/#3) either alone or on combination with ALDOA (sgALDOA #1, sgALDOA #2) in human liver cancer cells (HLE, HLF and SNU-387). Activation of AMPK was determined by analysing S79 phosphorylation on ACC. Vinculin is shown as loading control.

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