Extended Data Fig. 8: ALDOA depletion cannot be rescued by nucleoside addition.
a) Cell cycle distribution of control (shRen) or Aldo depleted (shAldoA.1280) Akt1myr;MycOE;Trp53–/– cells after 48 h of doxycycline treatment (1 µg/ml) followed by addition of fresh medium with or without nucleosides added for 6 h. Cells were incubated with 10 µM of BrdU for 0.5 h before harvest. b) Quantification of cell cycle distribution in control and AldoA depleted cells with or without addition of nucleosides. Data are presented as mean values ± SD. Significance was calculated using multiple two-tailed unpaired t-tests with Benjamini-Krieger-Yekutieli FDR correction (n = 3 biologically independent replicates, *p = 0.0075, **p = 0.0027, ***p = 0.00057, ****p = 0.0008, #p = 0.003, ##p = 0.0006). c) Proliferation of shRen and shAldoA Akt1myr;MycOE;Trp53–/– cells treated with doxycycline (1 µg/mL) and cultured with or without nucleosides. Data are displayed as mean ± SD (shRen n = 3, all others n = 4 parallel wells).
