Extended Data Fig. 1: In vitro screen identifies ALDOA as essential in HCC cells.
a) Distribution of individual shRNAs in the shRNA library used for the in vitro screen. b) A table showing the 10 top essential genes from the screen for each condition with essentiality scores. Full list is in supplementary table 2. c) Viability of Akt1myr;MycOE;Trp53–/– cells after 96hrs of AldoA depletion using the five shRNAs against AldoA from the screen. Data are presented as mean values ± SD. Significance was calculated using a two-tailed student’s t-test with FDR correction (n = 8 biologically independent replicates). d) Proliferation of control (shRen) or AldoA-depleted (shAldoA.1280) Akt1myr;MycOE;Trp53–/– cells in medium containing 1 µg/ml doxycycline (Dox). Data are presented as mean values ± SD (n = 3 biologically independent replicates). e) Representative images of control (shRen) or AldoA-depleted (shAldoA.1280) of Akt1myr;MycOE;Trp53–/– cells treated with 1 µg/ml doxycycline (Dox) for 48 h. f) Cell viability of Akt1myr;MycOE;Trp53–/– cells after knockout of Aldoa. Data are presented as mean values ± SD. Significance was calculated using one-way ANOVA with Dunnett’s post-hoc test (n = 3 biologically independent replicates).
