Extended Data Fig. 1: Impact of bilateral NG injection of OxtrCre mice with the Cre-inducible AAVPHP.S-FLEX-tdTomato or AAV5-hSyn-DIO-Gq-mCherry on fluorophore expression in the hindbrain.
From: Mechanosensation of the heart and gut elicits hypometabolism and vigilance in mice
(a) Representative confocal scans of coronal sections spanning the brainstem of OxtrCre mice that received a bilateral NG injection with the Cre-inducible AAVPHP.S-FLEX-tdTomato. (b) Corresponding atlas schematics of representative coronal sections highlighting the area postrema (AP), nucleus of the solitary tract (NTS), dorsal motor nucleus of the vagus (DMNV), and nucleus ambiguous (NA). (c) Representative coronal section depicting tdTomato-labeled fibers in the AP and NTS; however, the DMNV and NA are unlabeled. (d, left) Schematic of bilateral injection of AAVPHP.S-FLEX-tdTomato into the NG. (middle) Representative higher magnification images of the AP, NTS, DMNV, and (right) the NA. (e, left) Schematic of bilateral injection of AAV5-hSyn-DIO-mCherry into the NG. (middle) Representative higher magnification images of the AP, NTS, DMNV, (right) the and NA. CC; Central Canal, 4 V; 4th Ventricle. Scale Bars; 1 mm (a, c), 100 µm (d, e). n = 6-8 sections per 4 mice (tdTom groups) and 2-3 sections per 5 mice (Gq-mCherry groups). Schematics (d) and (e) made with Biorender.com.
