Extended Data Fig. 4: Kdm2a remodels lipid metabolism under HFD challenge.
From: Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity
(a) RT-qPCR analysis of glycolytic related gene expression in HFD-fed Kdm2a fl/fl and Kdm2aSKO male mice (n = 6 per group). (b) FFA levels in HFD-fed Kdm2a fl/fl and Kdm2aSKO male mice serum (n = 6 per group). (c) FFA levels in cold-adapted Kdm2a fl/fl and Kdm2aSKO male mice serum (n = 6 per group). (d) Western blot analysis of p-ACC/ACC and Cpt1a levels in Kdm2a-WT and Kdm2a-KO myotubes after PA stimulation (n = 4 per group). Protein quantitative analysis (right). (e) Western blot analysis of p-ACC/ACC and Cpt1a levels in Kdm2a-WT and Kdm2a-KO myotubes after 4°C stimulation (n = 4 per group). Protein quantitative analysis (right). Data were expressed as mean ± SEM. Student’s t-test was used for statistical analysis (a-e).
