Fig. 3: Loss of Kdm2a promotes lipid consumption in HFD-induced mice.
From: Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity
a, Body weight of HFD Kdm2afl/fl and Kdm2aSKO mice (n = 7 per group). b, Representative image of Kdm2afl/fl and Kdm2aSKO mice after 16 weeks of a HFD challenge (right) (n = 4 per group). c, Weight of fat and lean mass of HFD Kdm2afl/fl and Kdm2aSKO mice (n = 4 per group). d, Fasting blood glucose and e, serum insulin levels in Kdm2afl/fl and Kdm2aSKO mice after 16 weeks of HFD (n = 7 per group). f, Intraperitoneal glucose tolerance test and g, insulin tolerance test of Kdm2afl/fl and Kdm2aSKO mice after 16 weeks of HFD (n = 6 per group). h–k, Parameters measured in metabolic cages with HFD-fed Kdm2afl/fl and Kdm2aSKO mice: oxygen consumption and quantification in the right panel (h), Carbon dioxide emission and quantification in the right panel (i), average energy expenditure (j) and RER (k) (n = 8 per group). l, Random core body temperature at room temperature of HFD-fed Kdm2afl/fl and Kdm2aSKO mice (n = 5 per group). m, Core body temperature during fasting monitored with a cold challenge of HFD-fed Kdm2afl/fl and Kdm2aSKO mice (n = 5 per group). n, Representative image of HFD-fed Kdm2afl/fl (left) and Kdm2aSKO (right) mice Gas tissue (n = 5 per group). o, Western blot analysis of Myh4, Pgc1a and Myh7 in Gas tissues from Kdm2afl/fl and Kdm2aSKO mice fed with a HFD for 16 weeks (n = 4 per group). p, Representative immunostaining of Laminin (green), MHC I (red) and DAPI (blue) in Ta tissue cross sections from Kdm2afl/fl and Kdm2aSKO mice. Scale bars, 100 μm (n = 4 per group). q. Western blot showing expression of mitochondrial biogenesis-related proteins (Sdhb, Ndufs1, Ndufs3) in HFD-fed Kdm2afl/fl and Kdm2aSKO mice (n = 4 per group). r, Representative TEM images of Gas tissue cross sections from HFD-fed Kdm2afl/fl and Kdm2aSKO mice. Lipid droplets were indicated by arrows. Scale bars, 20 μm (n = 4 per group). Data are expressed as mean ± s.e.m. Statistical significance was assessed by two-way ANOVA (a, f–i, m) followed by Bonferroni’s multiple comparisons test or two-sided Student’s t-test (c–e, j–l, o, q).
