Fig. 4: Kdm2a remodels lipid metabolism under HFD challenge.
From: Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity
a,b, Gas tissues from HFD-fed Kdm2afl/fl and Kdm2aSKO male mice were isolated and subjected to deep RNA-seq analysis. KEGG pathways (a) and Gene Ontology (GO) analysis (b) were generated from RNA deep sequencing data (n = 3 per group). c, RT–qPCR analysis of the expression of lipolysis and fatty acid oxidation-related genes in HFD-fed Kdm2afl/fl and Kdm2aSKO male mice (n = 6 per group). d, RT–qPCR analysis of the expression of lipogenesis-related genes in HFD-fed Kdm2afl/fl and Kdm2aSKO male mice (n = 6 per group). e,f, Triglyceride (TG) and T-CHO levels in Gas tissues (e) and serum (f) of HFD-fed Kdm2afl/fl and Kdm2aSKO male mice (n = 6 or 7 per group). g, Representative western blot analysis of lipolysis-related proteins (phosphorylated ACC and Cpt1a) in HFD-fed Kdm2afl/fl and Kdm2aSKO male mice (n = 4 per group). h,i, TG and T-CHO levels in Gas tissues (h) and serum (i) of cold-adapted Kdm2afl/fl and Kdm2aSKO male mice (n = 6 per group). j, Representative western blot analysis of lipolysis-related proteins (phosphorylated ACC and Cpt1a) in cold-adapted male mice (n = 4 per group). Data are expressed as mean ± s.e.m. Two-sided unpaired Student’s t-test was used for statistical analysis (a, c–j).
