Fig. 6: Kdm2a could be a viable target against metabolic stress.
From: Kdm2a inhibition in skeletal muscle improves metabolic flexibility in obesity
a–d, Detection of oxygen consumption (a) and carbon dioxide emission (b), with quantification in the right panels, (c) average energy expenditure and (d) RER during light and dark conditions in metabolic cages (n = 8 per group). CON, PBS control; inhibitor, diaminozide treatment. e, Core body temperature of control and inhibitor groups (n = 7 per group) at 4 °C. f, Representative Gas photograph from control and inhibitor groups (n = 4 per group). g, Western blot (n = 4 per group) analysis of Myh4, Pgc1a and Myh7 expression in control and inhibitor groups. h, Representative immunostaining of Laminin (red), MHC I (green) and DAPI (blue) in frozen sections of control and inhibitor group Ta. Scale bars: 50 μm (n = 5 per group). i, Western blot analysis of Sdhb, Ndufs1, Ndufs3 expression in control and inhibitor groups (n = 4 per group). j, mtDNA copy number in control and inhibitor groups (n = 6 per group). k, Representative TEM images in control and inhibitor group Ta. Scale bars, 5 μm, n = 4 per group. l, RT–qPCR analysis of Ucp2, Ucp3, Ppara and Acadm expression in control and inhibitor group mice (n = 6 per group). m, Western blot analysis of p-ACC/ACC and Cpt1a levels in control and inhibitor group mice (n = 4 per group). n, TG and T-CHO content Gas tissue from control and inhibitor group mice (n = 8 per group). o, RT–qPCR analysis of Esrrg levels in Gas tissue of control and inhibitor group mice (n = 6 per group). p, Western blot measurement of H3K36me2, Kdm2a and Esrrg expression levels in control and inhibitor group mice (n = 4 per group). q, Relative RNA levels of transcribed exons 4–5 and intron 5 fragments in control and inhibitor group mice (n = 6 per group). Data are expressed as mean ± s.e.m. Statistical significance was assessed by two-way ANOVA (a, b, e) followed by Bonferroni’s multiple comparisons test or two-sided Student’s t-test (a–d, g–j, l–q).
