Extended Data Fig. 5: S-cyanylome remodeling caused by glycine or various cyanide donors.
From: Regulation of mammalian cellular metabolism by endogenous cyanide production
The proteome-wide and site-specific changes in S-cyanylation in (a) HepG2 cells treated with 10 nM KCN, (b) 100 µM mandelonitrile and (c) 100 µM amygdalin. N = 5/group, biological replicates. Colored dots represent cyanylated peptides found to be statistically significantly affected by the treatment; p < 0.05. (d) Venn diagram of proteins whose S-cyanylation was found to be significantly increased in HepG2 cells treated with 1 nM KCN, 10 nM KCN, and 100 µM mandelonitrile. (e) Proteome-wide and site-specific changes in S-cyanylation in HepG2 cells grown in glycine/serine free medium. (f) Annotated MS/MS spectra of peptide from glycine N-methyltransferase (UniProt accession: Q9QXF8) with C186 site either as carbamidomethylated (IAM) or containing light (blue L) or heavy (red H) tetrazole.
