Extended Data Fig. 8: Effect of KCN, glycine supplementation or serine/glycine deprivation on glycolysis and Krebs cycle.
From: Regulation of mammalian cellular metabolism by endogenous cyanide production
Metabolite levels analyzed by LCMS in HepG2 cells cultured in normal medium, in normal medium supplemented with 10 nM KCN, 10 mM glycine (Gly) or under deprivation of serine and glycine (-Ser/Gly). Relative % changes compared to the baseline levels of the metabolites (detected in normal culture medium) are shown. Metabolites in yellow squares were not detected by the method used. Data are expressed as the mean ± s.e.m. of n = 3 independent experiments and were analysed with a two-way ANOVA followed by Bonferroni’s multiple-comparisons test. *p < 0.05 and **p < 0.01 indicate significant differences.
