Extended Data Fig. 4: Elovl1-deficient CD8+ T cells show a rewired lipid profile.
a, Elovl1-7 expression quantified by in vitro bulk-RNA sequencing of sgNT and sgElovl1 OT-I T cells at day 7 from activation. The histogram shows the normalized read counts of Elovl family members. b, LS-MS lipidomics (n=5). Quantification of total lipid species. For each lipid species, the contribution of fatty acids with different chain lengths is shown. The statistic is applied to the total quantity of lipids in each species. SM= sphingomyelins; Cer= Ceramides; TG= triacylglycerides; DG= diacylglycerides; PC= phosphatidylcholine; PE= phosphatidylethanolamine; PG= phosphatidylglycerol; PI= phosphatidylinositol; PS= phosphatidylserine. c, Quantification of lipid droplets by the fluorescent probe BODIPY 493/503. Cells were incubated with the probe and MFI was quantified by flow cytometry (n=6). Data are presented as the mean ± s.e.m. Statistical significance was assessed by unpaired (b) or paired (c) two-tailed Student’s t-test.
