Extended Data Fig. 2: Asparaginase synergize with anti-PD-1 to elevate T cell antitumour responses.
(A) B16-F10-Luc-bearing C57BL/6 and Tcrb KO mice were administered with vehicle or L-asparaginase (5 U/g) three days before tumour inoculation and then 3 times per week by intraperitoneal injection. The average luminescent intensity of photons emitted from each tumour in the images was quantified. Data are presented as mean ± SEM (n = 4 mice per group). (B) B16-OVA-bearing C57BL/6 mice were administered with vehicle or L-asparaginase (5 U/g) or PBS on day 7 after tumour inoculation and received 1.5 × 10^6 activated OTI T cells on day 10. The average luminescent intensity of photons emitted from each tumour in the images was measured. Error bars represent the standard error of the mean (SEM). Data represent one independent experiment (n = 3 mice per group). Error bars indicate SEM. (C) MTCQ1-bearing C57BL/6 were treated with either a vehicle, L-ASP, anti-PD-1, or a combination of L-ASP and anti-PD-1 starting on day 7 after tumour inoculation. The average tumour volume is emitted from each tumour group (left) and final tumour weight at the endpoint of the experiment (right)(n = 4 mice per group). (D) Tumour weight was measured at the end of the experiment. The average tumour volume, luminescent intensity, and tumour weight for each treatment group are shown (n = 4). (E) B16-OVA-bearing C57BL/6 mice were treated with either a vehicle, L-ASP, anti-PD-1, or a combination of L-ASP and anti-PD-1 starting on day 7 after tumour inoculation (n = 4 mice per group). (F) Final tumour weight at the endpoint of the experiment (n = 4 mice per group). All data are mean ± SEM and were analysed by one-tailed unpaired (A, B, E, F) Student’s t-test, Sidak multiple comparisons tests (C) and Tukey’s multiple comparisons test (D) with adjusted P value and data are cumulative results from at least three independent experiments.
