Extended Data Fig. 7: Transcriptomics analysis of mouse colons in mice treated with and without 4HPAA.
a, The heat maps display a total of 42 upregulated genes and 64 downregulated genes. Genes related to lipid absorption and metabolism are marked with blue labels and the B cell immunity-related genes are marked with red labels. b, RT-PCR analysis shows that the colonic transcription of Cd36 and Scd1 was drastically inhibited in the 4HPAA-fed (for 3 months) mice compared to control mice (n = 5 mice per group). c, Representative DIC images of the mouse small intestinal (SI) organoids treated with or without 100 μM 4HPAA and 3HPP for 3 days. The scale bar represents 100 μm. d, The Cd36, Scd1, and Aqp7 transcripts from the organoids in (c) were quantified by RT-qPCR (n = 4 for the mock group and n = 3 for other groups). For b and d, error bars indicate mean ± SEM, two-tailed Mann-Whitney test, experiments were repeated twice independently with similar results.
