Extended Data Fig. 9: Compartments of B cells, T cells, Macrophages, DCs, NKs, and ILCs in mouse colons.

a, Frequency of memory B (n = 7 mice per group), follicular B (n = 7), CD4⁺ T (n = 8), CD8⁺ T (n = 8), Th17 (n = 4), Treg (n = 4), ILC1 (n = 5), ILC2 (n = 5), and ILC3 (n = 5) cell populations in the colon of HFD-fed mice treated with or without 4HPAA on day 16. Error bars indicate mean ± SEM, two-tailed Mann-Whitney test. b, Representative images of flow cytometric plots of B cells (CD45⁺B220⁺), follicular B cells (CD45⁺B220⁺IgA⁻CD21⁺CD23⁺), memory B cells (CD45⁺B220⁺IgA⁻CD38⁺GL7⁻IgM⁻IgD⁻), T cells (CD45⁺CD3⁺), CD4⁺ T (CD45⁺CD3⁺CD4⁺CD8⁻), CD8⁺ T (CD45⁺CD3⁺CD8⁺CD4⁻), Th17 cells (CD45⁺CD3⁺CD4⁺CD8⁻FOXP3⁻RORγt⁺), Tregs (CD45⁺CD3⁺CD4⁺CD8⁻FOXP3⁺), NK cells (CD45⁺CD3⁻NK1.1⁺), ILC cells (CD45⁺Lin⁻CD90.2⁺), ILC1 (CD45⁺Lin⁻CD90.2⁺KLRG1⁻RORγT⁻), ILC2 (CD45⁺Lin⁻CD90.2⁺KLRG1⁺RORγT⁻), ILC3 (CD45⁺Lin⁻CD90.2⁺RORγT⁺KLRG1⁻), DCs (CD45⁺CD11c⁺MHCII⁺CD64⁻), and Macrophages (CD45⁺CD11b⁺CD64⁺ SiglecF⁻).

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